Time required:

45min - 1h30min

Materials:

PBS Fibronectin:

• Stem Cell technologies 1mg/ml Cat#07159 (old one)
• Sigma Aldrich Cat#1141 1mg/ml link

Procedure:

1. Optional: sterilize surfaces by UV in the cell culture hood for 30min
2. Dilute Fibronectin in sterile PBS 1:100 to get 10ug/mL
3. Cover cultivation surface
4. Incubate at Room Temperature for 1h or 30 min at 37C
5. Aspirate before cell seeding

Notes:

• For hydrophobic surfaces (PDMS, Ibidi soft plates) use 0.5mL for 12well, 1mL for Ibidi plate
• For ibidi slides, use 200uL per well