Primers for cardio-characterization

These are the primers relevant for Cardio-Characterization Physically present in MBD-Brno:) Please keep updating it.
Can be searched and selected rows and columns exported in csv, pdf, and excel

Primers Table

Select columns and rows you need:

How to edit the table

Link to google sheet of the table is here.
To avoid a possibility to have it erased by some random person from internet google sheet for editing is invite only, please ask Vlada to share it with you.

Please add:

  • The sequences
  • melting temps and GC content
  • Reference (which paper it comes from)
  • Responsible (who designed the primer, used it, can help)

How to check sequence of primers for a given gene

Tutorial by Terka

download the video

How to check the primers we ordered from Eurofins

Eurofins have a good searchable history of our orders, please check before you buy your primers.
NOTE: Ask Honza or Vlada for the password, is written on the office door.

download the video

How to order primers

Log into the system

NOTE: Ask Honza or Vlada for the password, is also written on the office door.

Go into quick order

Select the format of primers

Select number of primers and sequences

Confirm and proceed to payment

To make sure we know who ordered what select your PI address or create a new one

Order

Notes

  • To add primers to the list, please ask Vlada for sharing the link to google table
  • “Collaborators only” is for the lab members with github account to modify the content of the page